Freddy Boutrot
Post Doctoral Scientist
Contact details
Email: freddy.boutrot@sainsbury-laboratory.ac.uk
Research interests
Plants respond to the perception of general elicitors or pathogen-associated molecular patterns (PAMP) by triggering the innate immune response. Within a few minutes, elicitor recognition results in the activation of several cellular responses, such as elevation of extracellular pH, depolarization of the plasma membrane, increase of cytoplasmic calcium concentration, production of nitric oxide, phosphatidic acid, and reactive oxygen species (ROS), activation of protein kinases such as mitogen-activated protein kinases (MAPK) and induction of defense gene expression.
I developed a screening assay based on monitoring flg22-induced ROS generation to identify Arabidopsis mutants deficient in the PAMP signalling pathway. I also use a reverse genetic approach (yeast-2 hybrid and in planta coIP) to investigate the possible involvement of candidate genes in this pathway.
I am currently involved in an ERA-PG program (PRR-CROP) to investigate pattern recognition receptors (PRR) in crops for durable disease control and identify new PRR in A. thaliana.
Selected Publications
BOUTROT F., SEGONZAC C., CHANG K.N., QIAO H., ECKER J.R., ZIPFEL C. and RATHJEN J.P. (2010)
Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1 Proc. Natl. Acad. Sci. USA 107(32):14502-14507 [Abstract]
BOUTROT F., CHANTRET N. and GAUTIER M.F. (2008)
Genome-wide analysis of the rice and Arabidopsis non-specific lipid transfer protein (nsLtp) gene families and identification of wheat nsLtp genes by EST data mining. BMC Genomics 9:86 [Abstract]
BOUTROT F., MEYNARD D., GUIDERDONI E., JOUDRIER P. and GAUTIER M.F. (2007)
The Triticum aestivum non specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice. Planta 225(4):843-862 [Abstract]
BOUTROT F., GUIRAO A., ALARY R., JOUDRIER P. and GAUTIER M.F. (2005)
Wheat non specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophys. Acta 1730(2):114-125 [Abstract]
MONNET F.P., DIERYCK W., BOUTROT F., JOUDRIER P. and GAUTIER M.F. (2001)
Purification, characterisation and cDNA cloning of a type 2 (7 kDa) lipid transfer protein from Triticum durum. Plant Sci. 161(4):747-755 [Abstract]
CUQ B., BOUTROT F., REDL A. and LULLIEN-PELLERIN V. (2000)
Study of the temperature effect on the formation of wheat gluten network: Influence on mechanical properties and protein solubility. J. Agric. Food Chem. 48:2954-2959 [Abstract]
